Laboratory Research in Biotechnology
Assessment Forms: Biotech Notebook Rubric, Project Proposal Form, Project Proposal Rubric, Project Progress Rubric,
PowerPoint Presentations: Transformation and pdf,Plasmid Miniprep pdf, pGlo cutting guidelines pdf version, Protein Purification and pdf, Restriction Analysis and pdf, Protein Electrophoresis and pdf, Western Blot and pdf, Real Time PCR and pdf,
Biotech 1Lab Protocols:
Bacterial Streak Plating,
Gram Staining,
pGlo Transformation,
Aurum Miniprep Kit,
Quantum Prep (miniprep),
Alkaline Lysis Miniprep, DNA
Quantitation by Electrophoresis, Restriction
Analysis,
Protein Purification,
Bradford Test for Proteins using a
Spectrophotometer,
Mitochondrial Sequencing, Protein
Quick Guide,
Protein Purification Quick Guide, HIV Elisa
Quick Guide
Biotech 2 and 3 Lab Protocols:
Ligation,
pKan Sequence,
pAmp Sequence,
pBR322 Sequence,
Competent Cell Production,
Electroporation,
Western Blot Quick Guide,
Western Blot Manual,
Primer
Hydrations, Primer Dilution and
Design, Real Time PCR Manual,
General Information and How To's: Course Syllabus, Lab Book Pointers, Lab Calcs, Dilutions, Molar Solutions, How to make a Gel, How to use a micropipet, semilog graphing, Dilutions review from Texas A&M, MSDS of Phenol, MSDS of Sodium Hydroxide, Graphing of Polyacrylamide Gel Electrophoresis, Poster Sample LLNL, Poster Sample THS
Useful websites:
Protocol Search:
Vadlo: Search Engine for Biologists. Find PPTs, Protocols, and other information
Restriction Enzyme Sites:
This is a site that allows students to make a plasmid map and virtually cut a plasmid and run a gel online. It will allow plasmid maps to be drawn and to make custom diagrams.
This site enables students to paste a sequence into a window and determine cut patterns.
Bioinformatics Sites:
This site is where software can be downloaded that can be used to visualize the protein structure with the use of protein X-Ray Crystallography structures.
This site has programs for looking at molecular structures, making movies, etc...
Online Tools:
This site converts from g to rpm or visa-versa. This is useful when you have the spin speeds given in g forces not revolutions per minute.
THis tool locates where your primers bind.
Primer Design (Primer3)
This tool is to design primers given a particular stretch of DNA.
This site has tools that can be used to design primers used for various tasks like SNPS etc..
This site will align both DNA and protein Sequences. It will also use that data to generate Cladograms.